DNA

Part:BBa_K1473002:Design

Designed by: Shan, Zhe   Group: iGEM14_TCU_Taiwan   (2014-09-17)


gRNA 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make sure our design is valid for CRISPR system. We screened raw result (all 20bp sequences upstream to PAM sites) by calculating it's GC account and testing its specificity by bioinformatic tools.


Source

It is designed from nalR gene from bacteria JM101's genome sequence. The design is under basic rules of gRNA design: 20bp upstream from PAM site.

References